Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures.
نویسندگان
چکیده
Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.
منابع مشابه
Large-scale purification of ribonucleic acid tumor viruses by use of continuous-flow density gradient centrifugation.
This report describes a 200- to 300-fold increase in the quantity of ribonucleic acud tumor virus particles previously isolated at one time by zonal centrifugation.
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عنوان ژورنال:
- Applied microbiology
دوره 24 3 شماره
صفحات -
تاریخ انتشار 1972